Rapid and specific detection of RNA base sequence using fluorescence polarization.
Identifieur interne : 000054 ( Ncbi/Merge ); précédent : 000053; suivant : 000055Rapid and specific detection of RNA base sequence using fluorescence polarization.
Auteurs : M. Tsuruoka [Japon] ; T. FujiiSource :
- Nucleic acids symposium series [ 0261-3166 ] ; 1999.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : RNA, Viral.
- chemical , genetics : RNA, Viral.
- chemical : DNA Probes, Oligodeoxyribonucleotides.
- genetics : Hepacivirus.
- methods : Fluorescence Polarization.
- Base Sequence, Genome, Viral, Molecular Sequence Data, Sensitivity and Specificity.
Abstract
Rapid and specific determination of the RNA gene of hepatitis C virus (HCV), which had been multiplied by NASBA, was performed using a fluorescence polarization assay. The polarization of the probe DNA in the presence of HCV positive sample, amplified by NASBA, was obviously different from those in the presence of negative control samples. The total time for the gene amplification and detection was about 90 min, while the polarization detection was completed within 10 min. The slight increase of polarization was also confirmed with the hybridization between probe oligo-DNA 25-mers and the synthesized complementary oligo-RNA 25-mers. The polarization of positive and negative samples showed excellent agreement with the results obtained from electrophoresis and dot-blot hybridization.
DOI: 10.1093/nass/42.1.239
PubMed: 10780468
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002591
- to stream PubMed, to step Curation: 002591
- to stream PubMed, to step Checkpoint: 002474
Links to Exploration step
pubmed:10780468Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rapid and specific detection of RNA base sequence using fluorescence polarization.</title><author><name sortKey="Tsuruoka, M" sort="Tsuruoka, M" uniqKey="Tsuruoka M" first="M" last="Tsuruoka">M. Tsuruoka</name><affiliation wicri:level="1"><nlm:affiliation>Advanced Science and Technology Laboratory, Hiroshima City, Hiroshima, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Advanced Science and Technology Laboratory, Hiroshima City, Hiroshima</wicri:regionArea><wicri:noRegion>Hiroshima</wicri:noRegion></affiliation></author><author><name sortKey="Fujii, T" sort="Fujii, T" uniqKey="Fujii T" first="T" last="Fujii">T. Fujii</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1999">1999</date><idno type="RBID">pubmed:10780468</idno><idno type="pmid">10780468</idno><idno type="doi">10.1093/nass/42.1.239</idno><idno type="wicri:Area/PubMed/Corpus">002591</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002591</idno><idno type="wicri:Area/PubMed/Curation">002591</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002591</idno><idno type="wicri:Area/PubMed/Checkpoint">002474</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002474</idno><idno type="wicri:Area/Ncbi/Merge">000054</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Rapid and specific detection of RNA base sequence using fluorescence polarization.</title><author><name sortKey="Tsuruoka, M" sort="Tsuruoka, M" uniqKey="Tsuruoka M" first="M" last="Tsuruoka">M. Tsuruoka</name><affiliation wicri:level="1"><nlm:affiliation>Advanced Science and Technology Laboratory, Hiroshima City, Hiroshima, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Advanced Science and Technology Laboratory, Hiroshima City, Hiroshima</wicri:regionArea><wicri:noRegion>Hiroshima</wicri:noRegion></affiliation></author><author><name sortKey="Fujii, T" sort="Fujii, T" uniqKey="Fujii T" first="T" last="Fujii">T. Fujii</name></author></analytic><series><title level="j">Nucleic acids symposium series</title><idno type="ISSN">0261-3166</idno><imprint><date when="1999" type="published">1999</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>DNA Probes</term><term>Fluorescence Polarization (methods)</term><term>Genome, Viral</term><term>Hepacivirus (genetics)</term><term>Molecular Sequence Data</term><term>Oligodeoxyribonucleotides</term><term>RNA, Viral (chemistry)</term><term>RNA, Viral (genetics)</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral ()</term><term>ARN viral (génétique)</term><term>Données de séquences moléculaires</term><term>Génome viral</term><term>Hepacivirus (génétique)</term><term>Oligodésoxyribonucléotides</term><term>Polarisation de fluorescence ()</term><term>Sensibilité et spécificité</term><term>Sondes d'ADN</term><term>Séquence nucléotidique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Probes</term><term>Oligodeoxyribonucleotides</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Hepacivirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Hepacivirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Fluorescence Polarization</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Genome, Viral</term><term>Molecular Sequence Data</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ARN viral</term><term>Données de séquences moléculaires</term><term>Génome viral</term><term>Oligodésoxyribonucléotides</term><term>Polarisation de fluorescence</term><term>Sensibilité et spécificité</term><term>Sondes d'ADN</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Rapid and specific determination of the RNA gene of hepatitis C virus (HCV), which had been multiplied by NASBA, was performed using a fluorescence polarization assay. The polarization of the probe DNA in the presence of HCV positive sample, amplified by NASBA, was obviously different from those in the presence of negative control samples. The total time for the gene amplification and detection was about 90 min, while the polarization detection was completed within 10 min. The slight increase of polarization was also confirmed with the hybridization between probe oligo-DNA 25-mers and the synthesized complementary oligo-RNA 25-mers. The polarization of positive and negative samples showed excellent agreement with the results obtained from electrophoresis and dot-blot hybridization.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">10780468</PMID><DateCompleted><Year>2000</Year><Month>06</Month><Day>29</Day></DateCompleted><DateRevised><Year>2019</Year><Month>11</Month><Day>03</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0261-3166</ISSN><JournalIssue CitedMedium="Print"><Issue>42</Issue><PubDate><Year>1999</Year></PubDate></JournalIssue><Title>Nucleic acids symposium series</Title><ISOAbbreviation>Nucleic Acids Symp. Ser.</ISOAbbreviation></Journal><ArticleTitle>Rapid and specific detection of RNA base sequence using fluorescence polarization.</ArticleTitle><Pagination><MedlinePgn>239-40</MedlinePgn></Pagination><Abstract><AbstractText>Rapid and specific determination of the RNA gene of hepatitis C virus (HCV), which had been multiplied by NASBA, was performed using a fluorescence polarization assay. The polarization of the probe DNA in the presence of HCV positive sample, amplified by NASBA, was obviously different from those in the presence of negative control samples. The total time for the gene amplification and detection was about 90 min, while the polarization detection was completed within 10 min. The slight increase of polarization was also confirmed with the hybridization between probe oligo-DNA 25-mers and the synthesized complementary oligo-RNA 25-mers. The polarization of positive and negative samples showed excellent agreement with the results obtained from electrophoresis and dot-blot hybridization.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tsuruoka</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Advanced Science and Technology Laboratory, Hiroshima City, Hiroshima, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fujii</LastName><ForeName>T</ForeName><Initials>T</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Nucleic Acids Symp Ser</MedlineTA><NlmUniqueID>8007206</NlmUniqueID><ISSNLinking>0261-3166</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015342">DNA Probes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009838">Oligodeoxyribonucleotides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="Y">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015342" MajorTopicYN="N">DNA Probes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005454" MajorTopicYN="N">Fluorescence Polarization</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016679" MajorTopicYN="N">Genome, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016174" MajorTopicYN="N">Hepacivirus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009838" MajorTopicYN="N">Oligodeoxyribonucleotides</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2000</Year><Month>4</Month><Day>26</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2000</Year><Month>7</Month><Day>6</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2000</Year><Month>4</Month><Day>26</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">10780468</ArticleId><ArticleId IdType="doi">10.1093/nass/42.1.239</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Fujii, T" sort="Fujii, T" uniqKey="Fujii T" first="T" last="Fujii">T. Fujii</name></noCountry><country name="Japon"><noRegion><name sortKey="Tsuruoka, M" sort="Tsuruoka, M" uniqKey="Tsuruoka M" first="M" last="Tsuruoka">M. Tsuruoka</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000054 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 000054 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Ncbi
|étape= Merge
|type= RBID
|clé= pubmed:10780468
|texte= Rapid and specific detection of RNA base sequence using fluorescence polarization.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:10780468" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |